Surveillance of current antibiotic resistance among clinical isolates S. aureus,E. coli and P. aeroginosa collected from five Iranian cities

Antibiotic resistance is an emerging problem worldwide present in many bacteria, speciallyS. aureus, P. aeroginosa, and E. coli that are consider as the most common group of bacteriaresponsible for nosocomial infections. This problem would be more serious and divesting indeveloping countries where there is not regular surveillance program for periodic antibioticresistance. In attempts to report the antibiotic pattern in Iranian cities, we collected clinicalisolates and tested them against current antibiotics. 653 Clinical isolates of S. aureus, P. aeroginosa,and E. coli were collected during 5 months. The isolates were then transported to researchlaboratory within glycerol containing medium. Standard antibiotic discs containing differentconcentrations of each agent were provided from MAST media. Standard disc diffusion methodwas carried out and the result was interpreted using NCCLS tables and data charts. Isolateswere mostly cultured from respiratory, Urine, wounds, blood clinical samples. The clinicalisolates were E. coli (45%) S. aureus (33%), and P. aeroginosa (22%). 20% of E. coli, 9% of P.aeroginosa, and 12% of S. aureus isolates showed reduction of sensitivity to Amikacin (AK).27% of S. aureus had reduction of sensitivity to Vancomycin (VA). About 25% of P. aeroginosashowed reduced sensitivity to Gentamicin (GM).